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AlphaLISA SureFire Ultra Human & Mouse Phospho-PLC-γ1 (Ser1248) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-PLC G1 (Ser1248) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-PLC G1 (Ser1248) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).

Offer EXTENDED - Save 10% on this product until September 23rd.

Promo code: SAVE2410
Part number: ALSU-PPLCG1-B-L
List price: USD 29.37
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USD 29.37
USD 29.37 /each

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G1 (Ser1248) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate A431 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 250 ng/mL EGF for 10 minutes and lysed in 4 mL of 1X SureFire Ultra Lysis buffer.

Specifications

Application
Protein Detection
Assay Target
PLC G1
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Experimental Type
In vitro
Shipping Conditions
Shipped in Blue Ice
Unit Size
1 Vial

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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