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AlphaLISA SureFire Ultra Human Total NRF2 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human Total NRF2 Detection Kit, 10,000 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Total Nrf2 assay is a sandwich immunoassay for quantitative detection of total Nrf2 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Total Nrf2 assay is a sandwich immunoassay for quantitative detection of total Nrf2 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Total NRF2 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human Total NRF2 Detection Kit, 10,000 Assay Points
Product information
Overview
Nuclear factor erythroid 2-related factor 2 (Nrf2), also known as nuclear factor erythroid-derived 2-like 2, is a basic leucine zipper (bZIP) encoded by the NFE2L2 gene. Based on preliminary research, Nrf2 may regulate the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. NRF2 is thought to play a pleiotropic function in the control of metabolism, inflammation, autophagy, proteostasis, mitochondrial physiology, and immunological responses. For the treatment of illnesses brought on by oxidative stress, numerous medications that promote the NRF2 pathway are being investigated.
The AlphaLISA SureFire Human Total Nrf2 Detection Kit is a sandwich immunoassay for the quantitative detection of total Nrf2 in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer B
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Nrf2
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Immuno-oncology
Inflammation
|
| Unit Size |
10,000 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Total NRF2 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human Total NRF2 Detection Kit, 10,000 Assay Points
Resources
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