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AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 50,000 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Phospho-NPM (Thr199) assay is a sandwich immunoassay for quantitative detection of phospho-NPM (Thr199) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Phospho-NPM (Thr199) assay is a sandwich immunoassay for quantitative detection of phospho-NPM (Thr199) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 50,000 Assay Points
Product information
Overview
Nucleophosmin (NPM, Nucleolar Phosphoprotein B23, No38, or Numatrin) is a highly conserved, abundant nucleolar protein found in the nuclei of proliferating cells. NPM is a chaperone protein that shuttles rapidly between the nucleus and cytoplasm. It plays key roles in ribosome biogenesis, centrosome duplication, genomic stability, cell cycle progression, and apoptosis. NPM overexpression, mutation, rearrangement, or deletion, is associated with several types of cancer.
The AlphaLISA SureFire Human Phospho-NPM (Thr199) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-NPM (Thr199) in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
NPM
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
50,000 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-NPM (Thr199) Detection Kit, 50,000 Assay Points
Resources
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