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AlphaLISA Surefire Ultra Total NFκB Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA SureFire Ultra total NFκB assay. This control is already provided in the AlphaLISA SureFire Ultra total NFκB assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Product information
Overview
Positive Control Lysate is prepared from HeLa cells, cultured to confluence in T-175 flasks in 10% FBS containing medium, then treated with 50 ng/mL TNFα plus 20 ng/mL Calyculin A for 10 minutes and lysed in 10 mL of 1X SureFire Ultra Lysis buffer.
Specifications
| Application |
Kinase
Protein Analysis & Detection
|
|---|---|
| Assay Target Class |
Protein
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Method |
Alpha
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Therapeutic Area |
Metabolic
|
| Unit Size |
1 vial
|
Video gallery
Resources
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
SDS, COAs, Manuals and more
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