Extra control lysate for the AlphaLISA SureFire Ultra phospho-NFκB (Ser536) assay. These controls are already provided in the AlphaLISA SureFire Ultra phospho-NFκB assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-NFκB p65 (Ser536) assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. This control lysate was prepared from HeLa cells, cultured to confluence in T175 flasks in 10% FBS containing medium for 3 days, then treated with 50 ng/mL TNFα and 20 ng/mL Calyculin A for 10 minutes and lysed in 10mL of 1X SureFire Ultra Lysis buffer.
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Target |
NFκB
|
Assay Target Class |
Phospho-protein
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
One Unit Contains |
1.0 each
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Metabolic
|
Unit Size |
1 vial
|
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