Extra control lysate for the AlphaLISA® SureFire® Ultra™ Total mTOR assay. This control is already provided in the AlphaLISA SureFire Ultra Total mTOR assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Total mTOR Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from Jurkat cells, cultured in a T175 flask to approximately 1 X 106 cells/mL in 10% FBS containing medium, then treated with 2 µg/mL EGF for 30 min and lysed at 1 X 107 cells/mL of 1X SureFire Ultra Lysis Buffer.
Application |
Protein Detection
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Unit Size |
1 vial
|
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