Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-PI3 Kinase p85 (Tyr458) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-PI3 Kinase p85 (Tyr458) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PI3 Kinase p85 (Tyr458) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate NIH/3T3 cells were cultured to 80-90% confluence in T175 flasks in 10% FBS containing medium, medium removed and then treated with 50 ng/mL PDGF-BB for 5 minutes and lysed in 5 mL of 1X SureFire Ultra Lysis buffer.
Application |
Protein Detection
|
---|---|
Assay Target |
PI3 Kinase p85
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Unit Size |
1 vial
|
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