Lymphocyte cytosolic protein 2 (SLP76) is a signal-transducing adaptor protein encoded by LCP2. This protein acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. Activated SLP76 translocates to the plasma membrane and promotes a multi-protein complex which participates in the activation, survival, and proliferation of T-Lymphocytes. SLP76 is associated with numerous therapeutic areas, including diseases such as multiple sclerosis, rheumatoid arthritis, psoriasis, and cancer.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-SLP76 (Ser376) assay is a sandwich immunoassay for the quantitative detection of phospho-SLP76 (Ser376) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening