Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total RIP3 (Mouse) assay. This control is already provided in the AlphaLISA SureFire Ultra Total RIP3 (Mouse) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Mouse Total RIP3 Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 2 hours at 37°C. Cells were lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
Application |
Protein Detection
|
---|---|
Assay Target |
RIP3
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Mouse
|
Therapeutic Area |
Autoimmunity
Central Nervous System
Neuroinflammation
|
Unit Size |
1 vial
|
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