Extra control lysate for the AlphaLISA SureFire Ultra mouse Phospho-MLKL (Ser345) assay. This control is already provided in the AlphaLISA SureFire Ultra mouse Phospho-MLKL (Ser345) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-MLKL (Ser345) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, L929 cells were cultured to confluence in T175 flasks in 10% FBS containing medium. Cells were treated with 20 μM Z-VAD, 100 nM SM-164 and 50 ng/mL TNFα for 4 hours at 37°C. Cells were lysed in 5 mL 1X SureFire Ultra Lysis buffer.
Application |
Protein Detection
|
---|---|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Therapeutic Area |
Inflammation
Neuroinflammation
Oncology
|
Unit Size |
1 Unit
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Are you looking for technical documents for this product. We have housed them in a dedicated section., click on the links below to explore.
We are here to answer your questions.