The AlphaLISA™ SureFire® Ultra™ mouse Phospho-MLKL (Ser345) assay is a sandwich immunoassay for quantitative detection of Phospho-MLKL (Ser345) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Mixed lineage kinase domain-like protein is encoded by the MLKL gene in murine species. MLKL is a pseudokinase that plays a crucial role in TNF-induced necroptosis. MLKL is activated following phosphorylation by RIP3 kinase, which induces a conformational switch essential for necroptosis. The protein kinase domain of MLKL is catalytically inactive, but contains a pseudoactive site with an interaction between Lys-219 and Gln-343 residues. Upon phosphorylation by RIP3, MLKL undergoes an active conformation and homodimerization through its amino-terminal coiled-coil domain.
The AlphaLISA™ SureFire® Ultra™ mouse p-MLKL (Ser345) assay is a sandwich immunoassay for quantitative detection of Phospho-MLKL (Ser345) in cellular lysates using Alpha Technology.
Formats:
AlphaLISA SureFire Ultra kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Protein Analysis & Detection
Protein Detection
|
---|---|
Assay Target |
MLKL
|
Assay Target Class |
Phospho-protein
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Inflammation
Neuroinflammation
Oncology
|
Unit Size |
10,000 Assay Points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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