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AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit, 10,000 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Total JAK1 assay is a sandwich immunoassay for quantitative detection of total JAK1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Total JAK1 assay is a sandwich immunoassay for quantitative detection of total JAK1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit, 10,000 Assay Points
Product information
Overview
JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases which include JAK2, JAK3, and TYK2. Numerous cytokines and growth factors, such as IL-6, IL-4, IFN-α, GM-CSF, induce the phosphorylation of JAKs. Activated JAK subsequently phosphorylates additional targets, including both cytokine receptors and major substrates, STATs. The JAK/STAT signaling pathway stimulates cell proliferation, differentiation, migration, and apoptosis. Alterations to JAK/STAT signaling reduce cytokine induced pro-inflammatory responses, making it an attractive target for anti-inflammatory therapies.
The AlphaLISA SureFire Human Total JAK1 Detection Kit is a sandwich immunoassay for the quantitative detection of total JAK1 in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
JAK1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Oncology
|
| Unit Size |
10,000 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit, 10,000 Assay Points
AlphaLISA SureFire Ultra Human Total JAK1 Detection Kit, 10,000 Assay Points
Resources
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This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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