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AlphaLISA SureFire Ultra Human & Mouse Total IRF5 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total IRF5 Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Total IRF5 assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Total IRF5 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human and Mouse Total IRF5 assay. This control is already provided in the AlphaLISA SureFire Ultra Human and Mouse Total IRF5 assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human & Mouse Total IRF5 Detection Kit, Control Lysate
AlphaLISA SureFire Ultra Human & Mouse Total IRF5 Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human and Mouse Total IRF5 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, THP-1 cells were seeded at 300K/mL, and incubated for 48 hours in T175 flasks in 10% FBS containing medium. Cells were harvested and resuspended at 8 x 106 cells/mL and treated with 100 nM Calyculin A for 3 hours. Cells were then washed in HBSS + 0.1% BSA and lysed in Lysis Buffer at a final cell density of 8 x 106 cells/mL.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Predicted Species Reactivity |
Human
Mouse
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRF5
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
Mouse
|
| Technology |
Alpha
|
| Therapeutic Area |
Inflammation
Oncology
|
| Unit Size |
1 Vial
|
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