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AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-IRAK4 (Thr345/Ser346) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRAK4 (Thr345/Ser346) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Human Phospho-IRAK4 (Thr345/Ser346) assay. This control is already provided in the AlphaLISA SureFire Ultra Human Phospho-IRAK4 (Thr345/Ser346) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human p-IRAK4 (Thr345/Ser346) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Karpas299 cells were seeded at 200K/mL and grown for two days in 100 mL of 10% FBS containing medium. Cells were harvested and washed in HBSS + 0.1% BSA, adjusted to 4 X 106 cells/mL and then treated with 10 ng/mL IL-1β for 10 minutes. Cells were spun down at 1000 g for 5 mins and lysed in 10 mL of SureFire Ultra Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
IRAK4
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Immuno-oncology
Inflammation
|
| Unit Size |
1 Vial
|
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AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-IRAK4 (Thr345/Ser346) Detection Kit, Control Lysate
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