AlphaLISA SureFire Ultra Human & Mouse Total IκBα Detection Kit, 100 Assay Points

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor α (IkBα) is a member of a family of cellular proteins that function to inhibit NF-kB transcription factor. When IkBα is phosphorylated by IKKβ, this prevents IkBα from inhibiting NF-kB which then frees NF-kB and allows it to enter the nucleus of the cell, where it activates various genes involved in inflammation and other immune responses. The IKKβ signaling pathway is associated with numerous therapeutic areas, including diseases such as multiple sclerosis, rheumatoid arthritis, psoriasis, and many types of cancers.

The AlphaLISA SureFire Ultra Human and Mouse Total IkBα Detection Kit is a sandwich immunoassay for the quantitative detection of total IkBα in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening