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AlphaLISA SureFire Ultra Human Total RIP3 Detection Kit, 500 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human Total RIP3 assay is a sandwich immunoassay for quantitative detection of total RIP3 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Part number: ALSU-TRIP3-A500
Unit Size: 500 Assay Points
List price: USD 2,319.00
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USD 2,319.00
USD 2,319.00 /each
Part number: ALSU-TRIP3-A10K
Unit Size: 10,000 Assay Points
List price: USD 14,270.00
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USD 0.00
USD 14,270.00 /each
Part number: ALSU-TRIP3-A50K
Unit Size: 50,000 Assay Points
List price: USD 40,710.00
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USD 0.00
USD 40,710.00 /each
Part number: ALSU-TRIP3-A-HV
Unit Size: 100 Assay Points
List price: USD 803.53
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USD 0.00
USD 803.53 /each

Overview

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3) is a critical regulator of programmed necroptosis, an inflammatory form of cell death with important functions in pathogen-induced and sterile inflammation. RIP3 mediates necroptosis in conjunction with RIPK1 and MLKL. Due to the inflammation caused by necroptosis, RIPK3 is associated with a variety of inflammatory diseases.

The AlphaLISA SureFire Ultra Human Total RIP3 assay is a sandwich immunoassay for the quantitative detection of total RIP3 in cellular lysates, using Alpha Technology.

Formats:
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for:
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Protein Detection
Assay Target
RIP3
Assay Technology
Alpha
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Method
Alpha
Shipping Conditions
Shipped in Blue Ice
Target Species
Human
Therapeutic Area
Autoimmunity
Central Nervous System
Neuroinflammation
Unit Size
500 Assay Points

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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