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AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-RIP3 (Ser227) (Human) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Ser227) (Human) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phospho-RIP3 (Ser227) (Human) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-RIP3 (Ser227) (Human) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate
Product information
Overview
This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Assay Kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, U937 cells were seeded at 800 K/mL and grown overnight in T175 flasks in 50 mL of 10% FBS containing medium. Cells were treated with 50 ng/mL TNFα, 20 μM Z-VAD and 200 nM SM-164 for 7 hours at 37°C. Cells were harvested, washed with HBSS and lysed in 5 mL of 1X SureFire Ultra Lysis Buffer.
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Control Lysate
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
RIP3
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Central Nervous System
Neuroinflammation
|
| Unit Size |
1 Vial
|
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AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate
AlphaLISA Surefire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, Control Lysate
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