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AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
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AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Phospho-RIP3 (Ser227) assay is a sandwich immunoassay for quantitative detection of phospho-RIP3 (Ser227) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Phospho-RIP3 (Ser227) assay is a sandwich immunoassay for quantitative detection of phospho-RIP3 (Ser227) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
Product information
Overview
Receptor-interacting serine/threonine-protein kinase 3 (RIPK3) is a critical regulator of programmed necroptosis, an inflammatory form of cell death with important functions in pathogen-induced and sterile inflammation. RIP3 mediates necroptosis in conjunction with RIPK1 and MLKL. Due to the inflammation caused by necroptosis, RIPK3 is associated with a variety of inflammatory diseases.
The AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) assay is a sandwich immunoassay for the quantitative detection of phospho-RIP3 (Ser227) in cellular lysates, using Alpha Technology.
Formats:
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
RIP3
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Autoimmunity
Central Nervous System
Neuroinflammation
|
| Unit Size |
500 Assay Points
|
Image gallery
AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
Video gallery
AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-RIP3 (Ser227) Detection Kit, 500 Assay Points
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