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AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Phospho-HSP27 (Ser78) assay is a sandwich immunoassay for quantitative detection of phopho-HSP27 (Ser78) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Phospho-HSP27 (Ser78) assay is a sandwich immunoassay for quantitative detection of phopho-HSP27 (Ser78) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points
Product information
Overview
Heat shock protein 27 (Hsp27) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a multidimensional protein which acts as a protein chaperone and an antioxidant and plays a role in the inhibition of apoptosis and actin cytoskeletal remodeling. In each of these capacities, HSP27 has been implicated in different disease states playing both protective and counter-protective roles. This protein has been indicated in renal injury, fibrosis, cancer, neurodegenerative disease, and cardiovascular disease.
The AlphaLISA SureFire Human Phospho-HSP27 (Ser78) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-HSP27 (Ser78) in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
HSP27
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Cardiovascular
Metabolic
Neuroscience
Oncology
|
| Unit Size |
500 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-HSP27 (Ser78) Detection Kit, 500 Assay Points
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