The AlphaLISA™ SureFire® Ultra™ p-GSK-3β (Ser9) assay is a sandwich immunoassay for quantitative detection of endogenous GSK-3β phosphorylated at Ser9 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
The AlphaLISA™ SureFire® Ultra™ p-GSK-3β (Ser9) assay is a sandwich immunoassay for quantitative detection of endogenous GSK-3β (phosphorylated at Ser9) in cellular lysates using Alpha Technology.
The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume. The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume. The 50,000 point kit contains reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
Application |
Kinase
Protein Analysis & Detection
|
---|---|
Assay Pathway |
PI3K/Akt/mTOR
|
Assay Target |
GSK3β
|
Assay Target Class |
Phospho-protein
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Format |
Microplates
|
Quantity in a Package Amount |
1.0 Units
|
Species |
Human
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Central Nervous System
Metabolic
|
Unit Size |
500 Assay Points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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