The AlphaLISA™ SureFire® Ultra™ Human and Mouse Total GSK3β assay is a sandwich immunoassay for quantitative detection of total GSK3β in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Glycogen synthase kinase-3 beta (GSK3β) is active in numerous central intracellular signaling pathways and regulates important cellular processes, from proliferation and immune response to glucose regulation and apoptosis. GSK3 kinase phosphorylation inhibits the activity of downstream targets such as β-catenin. GSK3 is regulated by cellular localization and by protein complex formation in the insulin pathway. Insulin activates PI3K, which phosphorylates AKT and in turn, activated AKT phosphorylates GSK3α on Ser21 and GSK3β on Ser9, resulting in GSK3 inactivation and inducing activation of glycogen synthase by dephosphorylation. GSK3 is considered a versatile target for various disease research applications, including oncology, neurological disorders, and diabetes.
The AlphaLISA SureFire Human and Mouse Total GSK3β Detection Kit is a sandwich immunoassay for the quantitative detection of total GSK3β in cellular lysates, using Alpha Technology.
Application |
Protein Analysis & Detection
Protein Detection
|
---|---|
Assay Target |
GSK3β
|
Assay Target Class |
Phospho-protein
|
Assay Technology |
Alpha
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Experimental Type |
In vitro
|
Shipping Conditions |
Shipped in Blue Ice
|
Target Species |
Human
Mouse
|
Therapeutic Area |
Metabolic
Neuroscience
Oncology
|
Unit Size |
100 Assay Points
|
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