Forkhead box protein M1 (FOXM1) is a transcription factor expressed throughout the cell cycle, rising in late G1-phase, reaching its apex in S-phase, and remaining there in G2/M and late M-phase. FOXM1 plays a significant role in multiple key cell cycle phases and is known as the master regulator of the cell cycle. The transcriptional network associated with FOXM1 regulates many processes, including stemness, cell survival, DNA damage repair, DNA replication, cell G1/S transition, cell cycle, cell proliferation, clonal formation, migration capacity, invasion, metastasis, and tumor initiation and progression. FOXM1 is found to be overexpressed in numerous malignancies such as breast, colorectal, hepatocellular, ovarian, lung, and pancreatic cancers.

The AlphaLISA SureFire Human Phospho-FOXM1 (Thr600) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-FOXM1 (Thr600) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening