AlphaLISA SureFire Ultra Human Phospho-FLT3 (Tyr591) Detection Kit, 10,000 Assay Points

Fms related receptor tyrosine kinase 3 (FLT3) is a member of the class III receptor kinase family. FLT3 acts as a cell surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. This kinase is involved in the activation of the downstream effort of MTOR, promotes activation of RAS signaling, and phosphorylates downstream kinases including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations of the FLT3 gene occur in approximately 30% of all acute myeloid leukemia cases.

The AlphaLISA SureFire Human Phospho-FLT3 (Tyr591) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-FLT3 (Tyr591) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening