AlphaLISA SureFire Ultra Human Phospho-FGF receptor 3 (Pan-Tyr) Detection Kit, 500 Assay Points

Fibroblast growth factor receptor 3 (FGFR3) is a transmembrane Receptor Tyrosine Kinase consisting of one intracellular tyrosine kinase domain. Upon ligand binding, the receptor dimerizes, and transphosphorylation of tyrosine kinase domains occurs. Activation of downstream signaling occurs via FRS2, which functions as a key adaptor protein, resulting in MAPK and PI3K/AKT activation. Genetic alteration of FGFR3 leads to constitutive activation of this receptor and has significant consequences in carcinogenesis, involved in lymphomas, urothelial, hepatocellular, lung, breast, and colorectal cancers.

The AlphaLISA SureFire Human Phospho-FGFR3 (pan-Tyr) Detection Kit is a sandwich immunoassay for the quantitative detection of Phospho-FGFR3 (pan-Tyr) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening