The AlphaLISA® SureFire® Ultra™ Total ERK 1/2 assay is a sandwich immunoassay for quantitative detection of ERK 1/2 (both phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be used as a normalization for phosphorylation studies.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
In the AlphaLISA®SureFire®Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA®SureFire®Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
Assay Pathway |
MAPK
|
---|---|
Assay Target Class |
Protein
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Format |
Microplates
|
One Unit Contains |
1.0 each
|
Quantity in a Package Amount |
500.0 Units
|
Shipping Conditions |
Shipped in Blue Ice
|
Therapeutic Area |
Cardiovascular
Central Nervous System
Neuroinflammation
|
Unit Size |
500 assay points
|
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
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