DNA-PK, a nuclear DNA-dependent serine/threonine protein kinase, consists of two components, a DNA-dependent protein kinase, catalytic subunit (DNA PKcs) and an autoimmune antigen (KU). DNA-PKcs is inactive and relies on KU to direct it to DNA ends and trigger kinase activity where it is required for the non-homologous end joining pathway of DNA repair which rejoins double-strand breaks. DNA repair genes likely underlie many forms of cancer, and reduced expression of DNA PKcs is associated with cancers such as breast, prostate, and gastric.

The AlphaLISA SureFire Human Total DNA PKcs Detection Kit is a sandwich immunoassay for the quantitative detection of total DNA PKcs in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening