DAP12, also known as TYRO protein (tyrosine kinase-binding protein), serves as a signaling adaptor for several cell surface receptors of the innate immune system. Crosslinking receptors related to DAP12 activate SYK or Zap-70 and other downstream targets, phosphorylate tyrosine residues in their cytoplasmic ITAM by SRC family kinases, and start downstream signaling. The TREM2-DAP12 receptor complex appears to be involved in maintaining a healthy brain homeostasis in microglia cells as well as monitoring the surroundings of neurons.

The AlphaLISA SureFire Ultra Mouse Total DAP12 Detection Kit is a sandwich immunoassay for the quantitative detection of total DAP12 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening