AlphaLISA SureFire Ultra Human DAP12 Aggregation Kit, 500 Assay Points

DAP12, also known as TYRO protein tyrosine kinase-binding protein, functions as a signaling adapter for a number of innate immune system cell surface receptors. By activating SYK or Zap-70 and other downstream targets, crosslinking receptors linked to DAP12 causes tyrosine residues in their cytoplasmic ITAM to be phosphorylated by SRC family kinases, which initiates downstream signaling. By monitoring the neuronal surroundings and preserving a healthy brain homeostasis in microglia cells, the TREM2-DAP12 receptor complex seems to play an ongoing role.

The AlphaLISA SureFire Human Aggregate DAP12 Detection Kit is a sandwich immunoassay for the quantitative detection of aggregate DAP12 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening