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AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 500 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Total Cyclin E1 assay is a sandwich immunoassay for quantitative detection of total cyclin E1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Total Cyclin E1 assay is a sandwich immunoassay for quantitative detection of total cyclin E1 in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 500 Assay Points
Product information
Overview
Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members have various roles in the cell cycle. CCNE1 forms a complex with cyclin-dependent kinase 2 (CDK2) and acts as a regulatory subunit. CDK2 is required for cell cycle G1/S transition. Overexpression of CCNE1 has been observed in many tumors, such as breast, ovarian, endometrial, and bladder cancer.
The AlphaLISA SureFire Human Total Cyclin E1 Detection Kit is a sandwich immunoassay for the quantitative detection of total Cyclin E1 in cellular lysates, using Alpha Technology.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Total
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Cyclin E1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
500 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Total Cyclin E1 Detection Kit, 500 Assay Points
Resources
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The cell cycle is tightly regulated by key proteins like Cyclin D1, which forms complexes to initiate progression through phases...
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