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AlphaLISA SureFire Ultra Human Phospho-Cyclin E1 (Thr77) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-Cyclin E1 (Thr77) Detection Kit, 50,000 Assay Points
The AlphaLISA™ SureFire® Ultra™ Human Phospho-Cyclin E1 (Thr77) assay is a sandwich immunoassay for quantitative detection of phospho-Cyclin E1 (Thr77) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ Human Phospho-Cyclin E1 (Thr77) assay is a sandwich immunoassay for quantitative detection of phospho-Cyclin E1 (Thr77) in cellular lysates using Alpha Technology.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-Cyclin E1 (Thr77) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-Cyclin E1 (Thr77) Detection Kit, 50,000 Assay Points
Product information
Overview
Cyclin E1, also known as CCNE1, is a member of the cyclin family, which is known for its various roles in the cell cycle. Cyclin E1 forms a complex with cyclin-dependent kinase 2 (CDK2), which is required for cell cycle G1/S transition, and acts as a regulatory subunit. Overexpression of Cyclin E1 is implicated in many tumors, including breast, ovarian, endometrial, and bladder cancer.
The AlphaLISA SureFire Human Phospho-Cyclin E1 (Thr77) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin E1 (Thr77) in cellular lysates, using Alpha Technology, with a cyclin E1 (phospho-Thr77) antibody that recognizes Cyclin E1 of human origin.
Formats
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire kits can be used for
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
Cyclin E1
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Therapeutic Area |
Oncology
|
| Unit Size |
50,000 Assay Points
|
Video gallery
AlphaLISA SureFire Ultra Human Phospho-Cyclin E1 (Thr77) Detection Kit, 50,000 Assay Points
AlphaLISA SureFire Ultra Human Phospho-Cyclin E1 (Thr77) Detection Kit, 50,000 Assay Points
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Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
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