Cyclin D3 is an atypical D-type cyclin that is primarily expressed in differentiated tissues. Overall, D-type cyclins (D1, D2, and D3) are involved in the regulation of transition from G1 to S during the cell cycle. Cyclin D activity is regulated through its mitogen-activated transcription, binding to CDK4/6, phosphorylation, ubiquitylation, nuclear export, and degradation. Cyclin D3 plays a key role in differentiation and maturation in lymphoid and musculoskeletal tissues. Increased expression of Cyclin D3 is associated with cells developing resistance to the CDK4 inhibitor Palbociclib, suggesting it as an effective therapeutic target.

The AlphaLISA SureFire Ultra Human Total Cyclin D3 Detection Kit is a sandwich immunoassay for the quantitative detection of total Cyclin D3 in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening