AlphaLISA SureFire Ultra Human & Mouse Total CREBBP Detection Kit, 500 Assay Points

The transcription coactivator CREB binding protein (CREBBP or CBP) is an acetyl transferase, that mediates histone 3 lysine 27 acetylation (H3K27ac) at regulatory elements such as enhancers and promoters. CREBBP/CBP inhibitors and degraders reduce H3K27ac, down-regulate oncogene transcription, induce cancer cell growth inhibition and cell death, activate immune response, overcome drug resistance and suppress tumor progression in vivo. CREBBP/CBP is over-expressed in cancer cells and in drug-resistant cancer cells, it activates oncogene transcription and induces cancer cell proliferation, survival, tumorigenesis, metastasis, immune evasion and drug-resistance.

The AlphaLISA SureFire Human and Mouse Total CREBBP Detection Kit is a sandwich immunoassay for the quantitative detection of total CREBBP in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening