The AlphaLISA™ SureFire® Ultra™ total CREB assay kit can be used to measure levels of CREB (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.
Feature | Specification |
---|---|
Application | Cell Signaling |
Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ total CREB assay kit can be used to measure levels of CREB (both phosphorylated and non-phosphorylated) in cellular lysates using no-wash Alpha technology.
Formats:
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
Alpha SureFire® kits can be used for:
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Total
|
Product Group |
Kit
|
Sample Volume |
10 µL
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
CREB
|
Target Class |
Phosphoproteins
|
Target Species |
Human
Mouse
|
Technology |
Alpha
|
Therapeutic Area |
Metabolic
|
Unit Size |
500 assay points
|
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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