Checkpoint kinase 2 (CHK2) is a serine-threonine kinase and an integral member of the signaling cascade that controls the G1 cell cycle checkpoint. CHK2 is activated in response to DNA double-strand breaks and phosphorylates several modulators of cell cycle control including tumor suppressor proteins and prevents unchecked cell division. Mutations in CHK2 are linked to a wide range of cancers, such as breast, lung, prostate, kidney, and thyroid.

The AlphaLISA SureFire Ultra Total CHK2 assay is a sandwich immunoassay for the quantitative detection of Total CHK2 in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening