Cyclin-dependent kinases 8 (CDK8) and its paralog, CDK19, are both commonly known as "Mediator Kinases," and are linked to developmental programming and cellular homeostasis. CDK8 and CDK19 are integrated into a 4-protein complex known as the Mediator kinase module. Reversibly, this module binds to the mediator, a protein complex consisting of 26 subunits that controls the expression of genes mediated by RNA Polymerase II. CDK8 is implicated in the development of various human diseases, including cancer.

The AlphaLISA SureFire Ultra Human and Mouse Total CDK8 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK8 in cellular lysates, using Alpha Technology.

 Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening