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AlphaLISA SureFire Ultra Human Total CDK6 Detection Kit, 10,000 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human Total CDK6 assay is a sandwich immunoassay for quantitative detection of total CDK6 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

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Feature Specification
Application Cell Signaling
Sample Volume 10 µL

The AlphaLISA™ SureFire® Ultra™ Human Total CDK6 assay is a sandwich immunoassay for quantitative detection of total CDK6 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Click to copy promo code to clipboard.
SAVE 15% NOW on online orders. Use promo code below.
YEAREND15
Offer valid until 12/15. Terms and conditions apply.
Product Variants
Unit Size: 500 Assay Points
Part #:
ALSU-TCDK6-A500
List Price
USD 2,346.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-TCDK6-A10K
List Price
USD 14,270.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-TCDK6-A50K
List Price
USD 46,060.00
Unit Size: 100 Assay Points
Part #:
ALSU-TCDK6-A-HV
List Price
USD 694.44

Overview

Cyclin-dependent kinase 6 (CDK6) is a member of the subfamily of CDKs that coordinate cell cycle progression in mammalian cells. Mitogenic signals, such as growth factors, trigger cells to enter the G1 phase of the cell cycle by inducing Cyclin D synthesis. Cyclin D then interacts with CDK4 and CDK6 to form active complexes. Both activated kinases are then able to mono-phosphorylate the tumor suppressor RB (protein of retinoblastoma), which still binds to transcription factor E2F, but some genes can be transcribed, such as Cyclin E. CDK6 is overexpressed in many cancers such as lymphoma, leukemia, glioblastoma, bladder, pancreas, and prostate.

The AlphaLISA SureFire Human Total CDK6 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK6 in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Total
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
CDK6
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
10,000 Assay Points

Video gallery

Resources

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

Application Note Icon
Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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