Cyclin-dependent kinase 5 (CDK5) is a proline-directed serine/threonine kinase that is a member of the Cyclin-dependent Kinase Family of proteins. Unlike other members of the family, the protein encoded by this gene does not only control cell cycle regulation, but rather mediates cell-cycle or DNA repairs through the phosphorylation of CK1. CDK5 activity reduces insulin secretion in pancreatic β cells and is implicated in IFNγ-induced PD-L1 upregulation, which allows certain cells to evade detection by the immune system and promotes the formation of lymphatic vessels. CDK5 is also associated with the migration of neurons and plays a role in the progression of various neurological diseases such as Alzheimer’s and Parkinson’s, as well as various types of cancers.

The AlphaLISA SureFire Human and Mouse Total CDK5 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK5 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening