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AlphaLISA SureFire Ultra Human Total CDK12 Detection Kit, 50,000 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human Total CDK12 assay is a sandwich immunoassay for quantitative detection of total CDK12 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling
Sample Volume 10 µL

The AlphaLISA™ SureFire® Ultra™ Human Total CDK12 assay is a sandwich immunoassay for quantitative detection of total CDK12 in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
ALSU-TCDK12-A500
List Price
USD 2,346.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-TCDK12-A10K
List Price
USD 14,270.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-TCDK12-A50K
List Price
USD 46,060.00
Unit Size: 100 Assay Points
Part #:
ALSU-TCDK12-A-HV
List Price
USD 694.44

Overview

Cyclin-dependent kinase 12 (CDK12) is a transcription-associated CDK. It complexes with Cyclin K to regulate gene transcription elongation via phosphorylating RNA polymerase II and regulates the expression of genes involved in DNA repair. CDK12 also plays a role in RNA splicing, cell cycle progression, cell proliferation, DNA damage response (DDR), and the maintenance of genomic stability. Mutation or amplification of CDK12 is closely related to tumorigenesis, associated with cancers such as breast, colorectal, liver, lung, ovarian, prostate, and bladder.

The AlphaLISA SureFire Human Total CDK12 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK12 in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Total
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
CDK12
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
50,000 Assay Points

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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