AlphaLISA SureFire Ultra Human Total CDK12 Detection Kit, 500 Assay Points

Cyclin-dependent kinase 12 (CDK12) is a transcription-associated CDK. It complexes with Cyclin K to regulate gene transcription elongation via phosphorylating RNA polymerase II and regulates the expression of genes involved in DNA repair. CDK12 also plays a role in RNA splicing, cell cycle progression, cell proliferation, DNA damage response (DDR), and the maintenance of genomic stability. Mutation or amplification of CDK12 is closely related to tumorigenesis, associated with cancers such as breast, colorectal, liver, lung, ovarian, prostate, and bladder.

The AlphaLISA SureFire Human Total CDK12 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK12 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening