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AlphaLISA SureFire Ultra Human and Mouse Phospho-CDK1 (Tyr15) Detection Kit, 10,000 Assay Points

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-CDK1 (Tyr15) assay is a sandwich immunoassay for quantitative detection of phospho-CDK1 (Tyr15) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling
Sample Volume 10 µL

The AlphaLISA™ SureFire® Ultra™ Human and Mouse Phospho-CDK1 (Tyr15) assay is a sandwich immunoassay for quantitative detection of phospho-CDK1 (Tyr15) in cellular lysates using Alpha Technology.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variants
Unit Size: 500 Assay Points
Part #:
ALSU-PCDK1-B500
List Price
USD 2,346.00
Unit Size: 10,000 Assay Points
Part #:
ALSU-PCDK1-B10K
List Price
USD 14,270.00
Unit Size: 50,000 Assay Points
Part #:
ALSU-PCDK1-B50K
List Price
USD 46,060.00
Unit Size: 100 Assay Points
Part #:
ALSU-PCDK1-B-HV
List Price
USD 694.44
USD 14,270.00
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Overview

Cyclin-dependent kinase 1 (CDK1), encoded by the CDC2 gene, is a serine/threonine kinase ubiquitously expressed in eukaryotes. CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis. Dysregulated activation of CDK1 leads to uncontrolled cell division and is a hallmark of cancer. Inhibition of CDK1 is an active research area, especially with new approaches involving PROTACs.

The AlphaLISA SureFire Human and Mouse Phospho-CDK1 (Tyr15) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-CDK1 (Tyr15) in cellular lysates, using Alpha Technology.

Formats
  • The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
  • The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
  • The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
  • The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.
AlphaLISA SureFire Ultra kits are compatible with
  • Cell and tissue lysates
  • Antibody modulators
  • Biotherapeutic antibodies
Alpha SureFire kits can be used for
  • Cellular kinase assays
  • Receptor activation studies
  • Screening

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Kit
Sample Volume
10 µL
Shipping Conditions
Shipped in Blue Ice
Target
CDK1
Target Class
Phosphoproteins
Target Species
Human
Mouse
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
10,000 Assay Points

Video gallery

Resources

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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Alpha SureFire Ultra no-wash immunoassay catalog

Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...

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Application Note
Characterizing Chemokine Receptor Inhibitors with AlphaLISA SureFire Ultra

Therapeutic antibodies directed to cell surface receptors are increasingly being developed as treatments for a variety of diseases...

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Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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Technical Note
Detecting changes in phosphorylation events during the cell cycle

Lamin A/C, a crucial nuclear protein, undergoes phosphorylation at mitotic sites, controlled by the CDK1-Cyclin B1 complex...

SDS, COAs, Manuals and more

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Safety data sheet
Certificate of analysis
Technical data sheet
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