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AlphaLISA SureFire Ultra Human Total CD3-ζ Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total CD3 ζ assay. This control is already provided in the AlphaLISA SureFire Ultra Total CD3 ζ assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Total CD3 ζ assay. This control is already provided in the AlphaLISA SureFire Ultra Total CD3 ζ assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variant
Unit Size: 1 Vial
Part #:
ALSU-TCD3-A-L
List Price
USD 142.66

Overview

This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total CD3 ζ Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, Jurkat cells were seeded at 500 K/mL and grown overnight in T175 flasks in 100 mL of 10% FBS containing medium. Cells were washed in HBSS + 0.1% BSA, adjusted to 3.5 X 106 cells/mL and treated with 44 mM H2O2 for 10 minutes. Cells were lysed with SureFire Ultra Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Total
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target
CD3 ζ
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Autoimmunity
Immuno-oncology
Oncology
Unit Size
1 Vial

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