Cluster of differentiation 3 Zeta (CD3 ζ) also known as T-cell receptor T3 zeta chain or CD247, is one of the four chains composing the CD3 complex. CD3 is a T-cell co receptor involved in activating both the cytotoxic T cell (CD8+ naive T cells) and T helper cells (CD4+ naive T cells). It is composed of two CD3ε chains, a CD3γ chain, and a CD3δ chain. The Zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response.

The AlphaLISA SureFire Ultra Human Phospho-CD3 ζ (Tyr142) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-CD3 ζ (Tyr142) in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening