c-Jun belongs to the mammalian Jun protein family which are transcription factors that must dimerize to bind to their target DNA sites. c-Jun is a major component of the activator protein 1 (AP-1) complex. Phosphorylation of c-Jun at Ser63 and Ser73 through JNK stimulates AP-1. Once phosphorylated, c-Jun can dimerize and bind to DNA sequences and affect transcription. c-Jun regulates numerous cellular responses including proliferation, malignant transformation, differentiation, and apoptosis. C-Jun plays a role in many types of cancers and autoimmune diseases.

The AlphaLISA SureFire Human and Mouse Phospho-c-Jun (Ser73) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-c-Jun (Ser73) in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening