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AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
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AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Phospho-Protein
The AlphaLISA™ SureFire® Ultra™ phospho-BTK (Tyr551) assay kit can be used to measure levels of BTK (phosphorylated at Tyr551) in cellular lysates using no-wash Alpha technology.
| Feature | Specification |
|---|---|
| Application | Cell Signaling |
| Sample Volume | 10 µL |
The AlphaLISA™ SureFire® Ultra™ phospho-BTK (Tyr551) assay kit can be used to measure levels of BTK (phosphorylated at Tyr551) in cellular lysates using no-wash Alpha technology.
Product Variants
Unit Size: 500 assay points
Part #:
ALSU-PBTK-B500
List Price
USD 2,392.92
Unit Size: 10,000 assay points
Part #:
ALSU-PBTK-B10K
List Price
USD 14,400.00
Unit Size: 50,000 assay points
Part #:
ALSU-PBTK-B50K
List Price
USD 46,000.00
Unit Size: 100 assay points
Part #:
ALSU-PBTK-B-HV
List Price
USD 708.33
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption, and disposal requirements under European REACH regulations (EC 1907/2006).
AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Phospho-Protein
AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
Product information
Overview
Formats:
- The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 µL reaction volume.
- The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume.
- The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume.
- The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 µL reaction volume.
In the AlphaLISA™ SureFire® Ultra™ assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of phosphorylated protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of phosphoprotein present in the sample.
AlphaLISA™ SureFire® Ultra™ kits are compatible with:
- Cell and tissue lysates
- Antibody modulators
- Biotherapeutic antibodies
Alpha SureFire® kits can be used for:
- Cellular kinase assays
- Receptor activation studies
- Screening
Specifications
| Application |
Cell Signaling
|
|---|---|
| Automation Compatible |
Yes
|
| Brand |
AlphaLISA SureFire Ultra
|
| Detection Modality |
Alpha
|
| Lysis Buffer Compatibility |
Lysis Buffer
|
| Molecular Modification |
Phosphorylation
|
| Product Group |
Kit
|
| Sample Volume |
10 µL
|
| Shipping Conditions |
Shipped in Blue Ice
|
| Target |
BTK
|
| Target Class |
Phosphoproteins
|
| Target Species |
Human
|
| Technology |
Alpha
|
| Unit Size |
500 assay points
|
Image gallery
AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
Video gallery
AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
AlphaLISA SureFire Ultra Human Phospho-BTK (Tyr551) Detection Kit, 500 Assay Points
Resources
Are you looking for resources, click on the resource type to explore further.
Guide
AlphaLISA SureFire Ultra assay optimization
This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...
Guide
AlphaLISA SureFire Ultra: the ultimate guide for successful experiments
The definitive guide for setting up a successful AlphaLISA SureFire Ultra assay
Several biological processes are regulated by...
Brochure
Alpha SureFire Ultra no-wash immunoassay catalog
Discover Alpha SureFire® Ultra™ assays, the no-wash cellular kinase assays leveraging Revvity's exclusive bead-based technology...
Application Note
Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
Flyer
Reagent solutions for autoimmunity research.
Advance your autoimmune disease research and benefit from Revvity broad offering of reagent technologies
Brochure
Species compatibility for HTRF, AlphaLISA SureFire Ultra and Alpha SureFire Ultra Multiplex assays
This document includes detailed tables listing HTRF™, AlphaLISA™ SureFire® Ultra™, and Alpha SureFire® Ultra™ Multiplex assays...
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