Bromodomain containing 7 (BRD7), also known as NAG4, BP75, or CELTIX1, is a member of the Bromodomain-containing Protein Family IV, which also includes BRD9. BRD7 plays a role in chromatin remodeling and regulation of transcription. Inhibition of BRD7 expression promotes cell proliferation and migration by activating ERK phosphorylation. Increasing evidence indicates that BRD7 is downregulated in many cancers, such as epithelial ovarian carcinoma, breast cancer, nasopharyngeal cancer, and colorectal carcinoma.

The AlphaLISA SureFire Human and Mouse Total BRD7 Detection Kit is a sandwich immunoassay for the quantitative detection of total BRD7 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening