Bromodomain-containing protein 4 (BRD4) is a member of the Bromodomains and Extraterminal (BET) family, consisting of BRD2, BRD3, and BRDT. BRD4 is a transcription regulator consisting of two bromodomains which bind acetylated lysine residues on proteins, such as histones. In association with Cyclin T1 and CDK9, BRD4 forms a complex called P-TEFb which controls gene transcription. BRD4 is also involved in DNA damage response, embryogenesis, and cancer development. Inhibiting BRD4 is a strategy for treating cancer, and more recently targeting BRD4 with PROTAC molecules has emerged as a novel therapeutic approach.

The AlphaLISA SureFire Human Total BRD4 Detection Kit is a sandwich immunoassay for the quantitative detection of total BRD4 in cellular lysates, using Alpha Technology.

Formats

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for

• Cellular kinase assays
• Receptor activation studies
• Screening