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AlphaLISA SureFire Ultra Human Phospho-Aurora B (Thr232) Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phopho-Aurora B (Thr232) assay. This control is already provided in the AlphaLISA SureFire Ultra Phopho-Aurora B (Thr232) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Feature Specification
Application Cell Signaling

Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phopho-Aurora B (Thr232) assay. This control is already provided in the AlphaLISA SureFire Ultra Phopho-Aurora B (Thr232) assay kit, but can also be ordered separately.

For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).

Product Variant
Unit Size: 1 Vial
Part #:
ALSU-PAURB-A-L
List Price
USD 142.66

Overview

This control lysate is intended for use with the AlphaLISA SureFire UltraHuman p-Aurora B (Thr232) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were grown to confluence in T175 flasks in 10% FBS containing medium, then treated with 50 nM Calyculin A and 2 μM PMA for 90 minutes at 37°C. Cells were lysed in 4 mL of SureFire Ultra Lysis Buffer.

Specifications

Application
Cell Signaling
Automation Compatible
Yes
Brand
AlphaLISA SureFire Ultra
Detection Modality
Alpha
Lysis Buffer Compatibility
Lysis Buffer
Molecular Modification
Phosphorylation
Product Group
Control Lysate
Shipping Conditions
Shipped in Blue Ice
Target
Aurora B
Target Class
Phosphoproteins
Target Species
Human
Technology
Alpha
Therapeutic Area
Oncology
Unit Size
1 Vial

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Guide
AlphaLISA SureFire Ultra assay optimization

This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible results are...

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