Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phopho-Aurora B (Thr232) assay. This control is already provided in the AlphaLISA SureFire Ultra Phopho-Aurora B (Thr232) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Cell Signaling |
Extra control lysate for the AlphaLISA™ SureFire® Ultra™ Phopho-Aurora B (Thr232) assay. This control is already provided in the AlphaLISA SureFire Ultra Phopho-Aurora B (Thr232) assay kit, but can also be ordered separately.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
This control lysate is intended for use with the AlphaLISA SureFire UltraHuman p-Aurora B (Thr232) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were grown to confluence in T175 flasks in 10% FBS containing medium, then treated with 50 nM Calyculin A and 2 μM PMA for 90 minutes at 37°C. Cells were lysed in 4 mL of SureFire Ultra Lysis Buffer.
Application |
Cell Signaling
|
---|---|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Modality |
Alpha
|
Lysis Buffer Compatibility |
Lysis Buffer
|
Molecular Modification |
Phosphorylation
|
Product Group |
Control Lysate
|
Shipping Conditions |
Shipped in Blue Ice
|
Target |
Aurora B
|
Target Class |
Phosphoproteins
|
Target Species |
Human
|
Technology |
Alpha
|
Therapeutic Area |
Oncology
|
Unit Size |
1 Vial
|
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