Ataxia-telangiectasia mutated (ATM) is a protein belonging to the P13/P14-kinase family. ATM is an important cell cycle checkpoint kinase and functions to regulate a wide variety of downstream proteins including p53, BRCA1, CHK2, RAD17, RAD9, and NBS1. ATM also plays a key role in double stranded DNA breaks and responds by phosphorylating histone H2AX, thus initiating a complex involving various other proteins that are recruited to the damage site. ATM mutations are associated with neurodegenerative disease and cancer.

The AlphaLISA SureFire UltraHuman Total ATM assay is a sandwich immunoassay for the quantitative detection of total ATM in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

Alpha SureFire kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• Screening