AlphaLISA SureFire Ultra Human and Mouse Phospho-α-Synuclein (Ser129) Aggregate Detection Kit, 100 assay points

Alpha-Synuclein (α-Syn) is a small, intrinsically disordered protein predominantly expressed in neurons, particularly in presynaptic terminals. It plays a crucial role in synaptic function, vesicle trafficking, neurotransmitter release and synaptic plasticity. In its normal state, α-Syn exists as a soluble monomer, but it can misfold and aggregate into oligomers, fibrils, and Lewy bodies. These aggregates are a hallmark of synucleinopathies, including Parkinson's disease (PD), Dementia with Lewy Bodies (DLB), and Multiple System Atrophy (MSA). Misfolded α-Syn disrupts cellular homeostasis, impairs synaptic function, and propagates toxic fibrils between neurons in a prion-like manner, leading to neurodegeneration. Therapeutic strategies aim to reduce α-Syn aggregation, enhance its degradation, and prevent its pathological spread.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-α-Synuclein (Ser129) Aggregate Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-α-Synuclein (Ser129) aggregate in cellular lysates, using Alpha Technology.

Formats:

• The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.
• The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.
• The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.
• The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

• Cell and tissue lysates
• Antibody modulators
• Biotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

• Cellular kinase assays
• Receptor activation studies
• High-throughput screening for preclinical studies