The AlphaLISA® SureFire®Ultra™ total AMPKα1/2 assay is a sandwich immunoassay for quantitative detection of AMPKα1/2 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. This assay is intended to be a normalization or control assay in conjunction with the AlphaLISA SureFire Ultra phospho-AMPKα1/2 assay.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
Formats:
In the AlphaLISA SureFire Ultra assay, Donor beads are coated with streptavidin to capture one of the antibodies, which is biotinylated. Acceptor beads are coated with a proprietary CaptSure™ agent that immobilizes the other antibody, labeled with a CaptSure™ tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample.
AlphaLISA SureFire Ultra™ kits are compatible with:
Alpha SureFire kits can be used for:
Application |
Protein Analysis & Detection
Target Engagement
|
---|---|
Assay Points |
500
|
Assay Target Class |
Protein
|
Assay Technology |
AlphaLISA SureFire Ultra
|
Automation Compatible |
Yes
|
Brand |
AlphaLISA SureFire Ultra
|
Detection Method |
Alpha
|
Therapeutic Area |
Cardiovascular
Central Nervous System
|
Unit Size |
500 assay points
|
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